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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/12/2018 |
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Data da última atualização: |
23/06/2022 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
SILVA-WERNECK, J. O.; BARROS, L. M. G.; NEVES, M. da S.; FALCAO, L. L.; MARCELLINO, L. H. |
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Afiliação: |
JOSEILDE OLIVEIRA SILVA WERNECK, Cenargen; LEILA MARIA GOMES BARROS, Cenargen; Michelly da Silva Neves, Estagiária da Embrapa Recursos Genéticos e Biotecnologia; LOENI LUDKE FALCAO, Cenargen; LUCILIA HELENA MARCELLINO, Cenargen. |
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Título: |
Transformação de tomate Micro-Tom com um gene tipo-PR de cupuaçuzeiro (Theobroma grandiflorum) visando o estudo de função da proteína. |
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Título original: |
Transformation of tomato Micro-Tom with a cupuassu (Theobroma grandiflorum) PR-like gene aiming the protein functional study. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Brasília-DF: Embrapa Recursos Genéticos e Biotecnologia, 2018. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 343) |
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Idioma: |
Português |
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Conteúdo: |
The cupuassu tree (Theobroma grandiflorum) is an Amazonian fruit species, whose production is highly affected by the fungus Moniliophthora perniciosa, which causes the witches? broom (WB) disease. Several genes potentially involved in the response to the pathogen have been identified in cupuassu, including some that code for PR (pathogenesis related)-like proteins. In this work, the gene TgPRa was selected for functional studies by heterologous expression in tomato Micro-Tom-Rg1 (MT-Rg1). This plant presents compatible interaction with M. perniciosa (biotype S), being a good model for WB studies. The TgPRa gene was cloned into pBI121, under the control of the Arabidopsis ACT2 promoter. The resulting vector, pBI121-TgPRa, also containing the gus reporter gene and the nptII selection gene, was inserted into Agrobacterium tumefaciens GV3101, which was used in the MT-Rg1 transformation. Kanamycin resistant and GUS-positive T0 plants, potentially transformed with the gene of interest, were obtained, what will be confirmed by PCR and Southern blot analysis. Plants are being grown to obtain MT-Rg1 homozygous for TgPRa. These plants will, in near future, be submitted to bioassays with M. perniciosa and other phytopathogenic fungi, aiming to evaluate the role of TgPRa in the development of WB, as well as other diseases, including some of interest to tomato cultivation. |
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Palavras-Chave: |
Gene PR; Tomate Micro-Tom; Tomateiro; Transformação; Transgênico. |
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Thesagro: |
Cupuaçu. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191048/1/Boletim-tomate-343.pdf
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Marc: |
LEADER 02364nam a2200253 a 4500
001 2102045
005 2022-06-23
008 2018 bl uuuu u0uu1 u #d
100 1 $aSILVA-WERNECK, J. O.
240 $aTransformation of tomato Micro-Tom with a cupuassu (Theobroma grandiflorum) PR-like gene aiming the protein functional study.
245 $aTransformação de tomate Micro-Tom com um gene tipo-PR de cupuaçuzeiro (Theobroma grandiflorum) visando o estudo de função da proteína.$h[electronic resource]
260 $aBrasília-DF: Embrapa Recursos Genéticos e Biotecnologia$c2018
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 343)
520 $aThe cupuassu tree (Theobroma grandiflorum) is an Amazonian fruit species, whose production is highly affected by the fungus Moniliophthora perniciosa, which causes the witches? broom (WB) disease. Several genes potentially involved in the response to the pathogen have been identified in cupuassu, including some that code for PR (pathogenesis related)-like proteins. In this work, the gene TgPRa was selected for functional studies by heterologous expression in tomato Micro-Tom-Rg1 (MT-Rg1). This plant presents compatible interaction with M. perniciosa (biotype S), being a good model for WB studies. The TgPRa gene was cloned into pBI121, under the control of the Arabidopsis ACT2 promoter. The resulting vector, pBI121-TgPRa, also containing the gus reporter gene and the nptII selection gene, was inserted into Agrobacterium tumefaciens GV3101, which was used in the MT-Rg1 transformation. Kanamycin resistant and GUS-positive T0 plants, potentially transformed with the gene of interest, were obtained, what will be confirmed by PCR and Southern blot analysis. Plants are being grown to obtain MT-Rg1 homozygous for TgPRa. These plants will, in near future, be submitted to bioassays with M. perniciosa and other phytopathogenic fungi, aiming to evaluate the role of TgPRa in the development of WB, as well as other diseases, including some of interest to tomato cultivation.
650 $aCupuaçu
653 $aGene PR
653 $aTomate Micro-Tom
653 $aTomateiro
653 $aTransformação
653 $aTransgênico
700 1 $aBARROS, L. M. G.
700 1 $aNEVES, M. da S.
700 1 $aFALCAO, L. L.
700 1 $aMARCELLINO, L. H.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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